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Expression of plasminogen activators <t>uPA</t> <t>and</t> <t>tPA</t> in normal and PCOS mouse ovaries. Left: Top: ( a ) uPA and Bottom: ( b ) tPA. Representative histological images are from normal (control) (Ai, Aiii, Bi, Biii) or PCOS ovaries (Aii, Aiv, Bii, Biv) stained with an ( a ) anti-uPA or ( b ) anti-tPA antibody. The scale bar represents 500 μm for Ai, Aii, Bi & Bii, 200 μm for Aiii & Biv and 100 μm for Aiv & Biii. Right: Top: Negative control for uPA on mouse kidney and graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for uPA between normal and PCOS ovaries for 6 replicates of each experimental group (mean ± SD) and Bottom: Negative control for tPA on mouse brain and graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for tPA between normal and PCOS ovaries for 6 replicates of each experimental group (mean ± SD)
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Expression of plasminogen activators <t>uPA</t> <t>and</t> <t>tPA</t> in normal and PCOS mouse ovaries. Left: Top: ( a ) uPA and Bottom: ( b ) tPA. Representative histological images are from normal (control) (Ai, Aiii, Bi, Biii) or PCOS ovaries (Aii, Aiv, Bii, Biv) stained with an ( a ) anti-uPA or ( b ) anti-tPA antibody. The scale bar represents 500 μm for Ai, Aii, Bi & Bii, 200 μm for Aiii & Biv and 100 μm for Aiv & Biii. Right: Top: Negative control for uPA on mouse kidney and graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for uPA between normal and PCOS ovaries for 6 replicates of each experimental group (mean ± SD) and Bottom: Negative control for tPA on mouse brain and graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for tPA between normal and PCOS ovaries for 6 replicates of each experimental group (mean ± SD)
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Expression of plasminogen activators <t>uPA</t> <t>and</t> <t>tPA</t> in normal and PCOS mouse ovaries. Left: Top: ( a ) uPA and Bottom: ( b ) tPA. Representative histological images are from normal (control) (Ai, Aiii, Bi, Biii) or PCOS ovaries (Aii, Aiv, Bii, Biv) stained with an ( a ) anti-uPA or ( b ) anti-tPA antibody. The scale bar represents 500 μm for Ai, Aii, Bi & Bii, 200 μm for Aiii & Biv and 100 μm for Aiv & Biii. Right: Top: Negative control for uPA on mouse kidney and graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for uPA between normal and PCOS ovaries for 6 replicates of each experimental group (mean ± SD) and Bottom: Negative control for tPA on mouse brain and graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for tPA between normal and PCOS ovaries for 6 replicates of each experimental group (mean ± SD)
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Expression of plasminogen activators <t>uPA</t> <t>and</t> <t>tPA</t> in normal and PCOS mouse ovaries. Left: Top: ( a ) uPA and Bottom: ( b ) tPA. Representative histological images are from normal (control) (Ai, Aiii, Bi, Biii) or PCOS ovaries (Aii, Aiv, Bii, Biv) stained with an ( a ) anti-uPA or ( b ) anti-tPA antibody. The scale bar represents 500 μm for Ai, Aii, Bi & Bii, 200 μm for Aiii & Biv and 100 μm for Aiv & Biii. Right: Top: Negative control for uPA on mouse kidney and graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for uPA between normal and PCOS ovaries for 6 replicates of each experimental group (mean ± SD) and Bottom: Negative control for tPA on mouse brain and graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for tPA between normal and PCOS ovaries for 6 replicates of each experimental group (mean ± SD)
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Expression of plasminogen activators <t>uPA</t> <t>and</t> <t>tPA</t> in normal and PCOS mouse ovaries. Left: Top: ( a ) uPA and Bottom: ( b ) tPA. Representative histological images are from normal (control) (Ai, Aiii, Bi, Biii) or PCOS ovaries (Aii, Aiv, Bii, Biv) stained with an ( a ) anti-uPA or ( b ) anti-tPA antibody. The scale bar represents 500 μm for Ai, Aii, Bi & Bii, 200 μm for Aiii & Biv and 100 μm for Aiv & Biii. Right: Top: Negative control for uPA on mouse kidney and graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for uPA between normal and PCOS ovaries for 6 replicates of each experimental group (mean ± SD) and Bottom: Negative control for tPA on mouse brain and graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for tPA between normal and PCOS ovaries for 6 replicates of each experimental group (mean ± SD)
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Expression of plasminogen activators <t>uPA</t> <t>and</t> <t>tPA</t> in normal and PCOS mouse ovaries. Left: Top: ( a ) uPA and Bottom: ( b ) tPA. Representative histological images are from normal (control) (Ai, Aiii, Bi, Biii) or PCOS ovaries (Aii, Aiv, Bii, Biv) stained with an ( a ) anti-uPA or ( b ) anti-tPA antibody. The scale bar represents 500 μm for Ai, Aii, Bi & Bii, 200 μm for Aiii & Biv and 100 μm for Aiv & Biii. Right: Top: Negative control for uPA on mouse kidney and graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for uPA between normal and PCOS ovaries for 6 replicates of each experimental group (mean ± SD) and Bottom: Negative control for tPA on mouse brain and graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for tPA between normal and PCOS ovaries for 6 replicates of each experimental group (mean ± SD)
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Innovative Research Inc rabbit anti mouse/rat urokinase (upa) polyclonal fractionated,rabbit anti-mouse urokinase igg fraction
Expression of plasminogen activators <t>uPA</t> <t>and</t> <t>tPA</t> in normal and PCOS mouse ovaries. Left: Top: ( a ) uPA and Bottom: ( b ) tPA. Representative histological images are from normal (control) (Ai, Aiii, Bi, Biii) or PCOS ovaries (Aii, Aiv, Bii, Biv) stained with an ( a ) anti-uPA or ( b ) anti-tPA antibody. The scale bar represents 500 μm for Ai, Aii, Bi & Bii, 200 μm for Aiii & Biv and 100 μm for Aiv & Biii. Right: Top: Negative control for uPA on mouse kidney and graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for uPA between normal and PCOS ovaries for 6 replicates of each experimental group (mean ± SD) and Bottom: Negative control for tPA on mouse brain and graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for tPA between normal and PCOS ovaries for 6 replicates of each experimental group (mean ± SD)
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Image Search Results


Expression of plasminogen activators uPA and tPA in normal and PCOS mouse ovaries. Left: Top: ( a ) uPA and Bottom: ( b ) tPA. Representative histological images are from normal (control) (Ai, Aiii, Bi, Biii) or PCOS ovaries (Aii, Aiv, Bii, Biv) stained with an ( a ) anti-uPA or ( b ) anti-tPA antibody. The scale bar represents 500 μm for Ai, Aii, Bi & Bii, 200 μm for Aiii & Biv and 100 μm for Aiv & Biii. Right: Top: Negative control for uPA on mouse kidney and graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for uPA between normal and PCOS ovaries for 6 replicates of each experimental group (mean ± SD) and Bottom: Negative control for tPA on mouse brain and graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for tPA between normal and PCOS ovaries for 6 replicates of each experimental group (mean ± SD)

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Expression of the plasminogen system in the physiological mouse ovary and in the pathological polycystic ovary syndrome (PCOS) state

doi: 10.1186/s12958-019-0472-0

Figure Lengend Snippet: Expression of plasminogen activators uPA and tPA in normal and PCOS mouse ovaries. Left: Top: ( a ) uPA and Bottom: ( b ) tPA. Representative histological images are from normal (control) (Ai, Aiii, Bi, Biii) or PCOS ovaries (Aii, Aiv, Bii, Biv) stained with an ( a ) anti-uPA or ( b ) anti-tPA antibody. The scale bar represents 500 μm for Ai, Aii, Bi & Bii, 200 μm for Aiii & Biv and 100 μm for Aiv & Biii. Right: Top: Negative control for uPA on mouse kidney and graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for uPA between normal and PCOS ovaries for 6 replicates of each experimental group (mean ± SD) and Bottom: Negative control for tPA on mouse brain and graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for tPA between normal and PCOS ovaries for 6 replicates of each experimental group (mean ± SD)

Article Snippet: The slides were incubated with the primary antibody at room temperature for either 90 min for PAI-1 (rabbit polyclonal antibody to mouse PAI-1, ABCAM: ab28207) (1:250), uPA (rabbit monoclonal antibody to mouse uPA, ABCAM: ab133563) (1:150), tPA (rabbit polyclonal antibody to mouse tPA, ABCAM: ab28208) (1:200) and plasminogen/plasmin antibodies (rabbit polyclonal antibody to mouse plasminogen/plasmin, Novus Biologicals: NBP2–19859) (1:300) or overnight at 4 °C for the plasminogen only antibody (rabbit polyclonal antibody to mouse plasminogen, Novus Biologicals: NB300–544) (1:100).

Techniques: Expressing, Staining, Negative Control